Q. Which stain shows Gram negative bacteria during bacterial staining?
Solution:
- Gram staining differentiates bacteria into two large groups: Gram-positive bacteria and Gram-negative bacteria by the chemical and physical properties of their cell walls.
- Gram-positive cells have a thick layer of peptidoglycan in the cell wall that retains the primary stain, crystal violet.
- Gram-negative cells have a thinner peptidoglycan layer that allows the crystal violet to wash out on addition of ethanol.
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