Q. After ethanol treatment Gram negative bacteria can be visualised
Solution:
- In Gram staining, the decolorizing agent, usually ethanol is added to the solution which consists of cells.
- Cells that have thick peptidoglycan layers in their cell walls are much less affected by the decolorizing agent; they generally retain the crystal violet dye and remain purple.
- However, the decolorizing agent more easily washes the dye out of cells with thinner peptidoglycan layers, making them colorless.
- Finally, a secondary counterstain, usually safranin, is added.
- This stains the decolorized cells pink.
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